EVALUATION OF ETHANOLIC ROOT EXTRACT OF ALLIUM CEPA. L FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES IN ANIMAL MODELS

  • Krupavaram. B School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.
    kruparajs@gmail.com
  • Kaashmeera Nair. K School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.
  • Hanish Singh. JC School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.
  • Anandarajagopal K School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.
  • Jaasminerjiit Kaur School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.
  • Shopana Paramasivam School of Pharmacy, KPJ Healthcare University College, Perserian Seriemas, Kota Seriemas, Nilai 71800, Negeri Sembilan, Malaysia.

Abstract

 Objectives: This present study was envisaged to identify the effect of analgesic and anti-inflammatory activity forethanolic root extract of Allium cepa L. Methodology: The study was carried out using Sprague- Dawley rats (250- 300g) and Albino mice (25- 30g). Diclofenac sodium and Tramadol are the standard drugs, was prepared by dissolving in distilled water to make the concentration of 10mg/kg and 20mg/kg for anti-inflammatory and analgesic activity respectively. The effect of ethanolic extract of A.cepa L root was investigated for analgesic activity using tail immersion method. The anti-inflammatory activity of ethanolic root extract of A.cepa was studied using carrageenan-induced paw edema to the rats.Results: The anti-inflammatory activity was evaluated using carrageenan method in Sprague- Dawley rats. The anti-inflammatory activity was found to be dose dependent in carrageenan induced paw edema model. The ethanolic extract has shown highly significant (p<0.001) percentage of inhibition of paw edema, 57.43% and 60.56% at 5th hour at the low dose and high dose, respectively. Whereas analgesic activity was studied using tail immersion method in mice. The ethanolic root extract was shown highly significant (p<0.001) analgesic activity with standard drug and high dose but, low dose showed less significant (p<0.05) analgesic activity. Conclusions: The findings of the present study concluded that A.cepa L roots have potential to treat pain and inflammation and as a good source, novel natural analgesic and anti-inflammatory agents. The ethanolic extract of A.cepa L root showed highly significant analgesic and anti-inflammatory activity in mice and rats respectively.

Keywords: Allium cepa L, Analgesic, Anti-inflammatory, Tail immersion, and Carrageenan

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References

1. Ayyanna Chakali, Sekar Munuswamy, Niranjan kumar Raghupathi, Ravi kumar Kota, Antidiabetic activity of Ethonolic extract of Allium cepa seeds against Alloxan induced Dieabetic rats.Journal of comprehensive pharmacy.2016,3(3)
2. Anar Patel, Timir Patel, Carol Macwan, Mayuree Patel, Khushbu Chauhan , Jatin Patel. Evaluation of Anti inflammatory and Analgesic activity of roots of Rubia cordifolia in rats. Journal pf Pharmaceutical Sciences and Research.2010;2 (12): 809-813.
3. Allium cepa Linn. (n.d.). IndianMedicinal Plants,1-1.doi:10.1007/978-0-387-70638-2_81
4. AJaykumar, T.V., Anandarajagopal, K., Anbu Jeba Sunilson, J. Adibah, A., Jainaf, R.A.M and Venkateshan,N.Anti-inflammatory activity of cosmos caudatus, International Journal of Universal Pharmacy and Bio Sciences.2012;1(2): 40-48.
5. Ajoy, K.G., shankhajit, D.E and Yadu, N.D. Anti-inflammatory activity of methanolic extract Ofamorphophallus pauoniifolius and its possible mechanism. International Journal of Chemical and Pharmaceutical Research.2010;7:16-12.
6. Anikumar M. Ethnomedicinal plants as anti-inflammatory and analgesic agents. In: Chattopadhyay D, editor. Etnomedicine: a source of complementary therapeutics.; Research signpost;2010: 267-293.
7. Ayyanna Chakali, Sekar Munuswamy, Niranjan kumar Raghupathi, Ravi kumar Kota. Antidiabetic activity of Ethonolic extract of Allium cepa seeds against Alloxan induced Diabetic rats. Journal of comprehensive pharmacy - 2016; 3( 3)
8. Debashree, N., Subhalakshmi.A Rita.S,and Pfuzia, A.Study of analgesic and anti-inflammatory effects of Impatients balsamina leaves in albino rats. International Journal of Pharma and Bio Sciences. 2013;4(2): 581-587.
9. Dorsch, W., E. Schneider, T. Bayer, W. Breu and H. Wagner “Anti-inflammatory effects of onions: inhibition of chemotaxis of human polymorphonuclear leukocytes by thiosulfinates and cepaenes." Int Arch Allergy Appl Immunol.1990;92(1): 39-42.
10. Dr. Jagdev Singh “Onions health benefits are due to its potent anti-oxidant, immunomodulatory and anti-inflammatory” : ayurtimes;2015.
11. FreddyA.Ramos,Y.T.,*,Miki Shirotori,Yousuke Kawaguchi,Koichiro Tsuchiya,Hirofumi Shibata,Tomihiko Higuti,Tetsuo Tadokoro,And Minoru Takeuchi."Antibacterial and Antioxidant Activities of Quercetin Oxidation Products from Yellow Onion (Allium cepa) Skin."J. Agric. Food Chem. 2006;54: 3551−3557.
12. Giresha, A.S., Narayanappa, M., Vikram, J., Vishwanath, B.S. and Dharmappa, K.K.Human Secretory phospholipase A2 (sPLA2) Inhibition by water extract of Macrotyloma uniflorum (seed) as an Anti-inflammatory activity. International journal of Pharmacy and Pharmaceutical Sciences.2015;7 (1):217-222
13. Rupa Sengupta, Sonali D Sheorey , Madhuri A Hinge.Analgesics and anti-inflammatoryplants. 2012;12( 2):114-11
14. Hemalini,K.K., Naik, O.P. and Ashok,P. Anti-inflammatory and Analgesic effect of methanolic extract of Anogessues acuminate leaf. International Journal Pharmaceutical iomedical Research.2010;1(3):98-101.
15. James B. Perianayagem, S.k. Sharma, Aney Joseph, A.J.M. Christina.Evaluation of Anti-Pyretic and Analgesic activity of Emblica Officinalis Gaertn. Journal of Ethno-pharmacology.2004;95.83-85.
16. Joseph BS1, Kumbhare PH and Kale MC. Preliminary phytochemical screening of selected Medicinal Plants.Int. Res. J. of Science & Engineering, 2013; 1(2): 55-62.
17. Khaki, F. Fathiazad M. N ouri, A.A. Khaki , H.J. Khamenehi, M. Hamadeh .Evaluation of androgenic activity of Allium cepa on spermatogenesis in the rat. Via Meidca.2008; 68(1): 45–51.
18. Kumi Kaneko, Masato Umehara, Takashi Homan, Ken Okamota, Michiko oKa, Tatsuya Oyama. The Analgesic Effect of Tramadol in animal models of neuropathic pain and fibromyalgia. Neuroscience Letters.2014; 562:28-33.
19. Mohamad Ali Hijazi, Ahmed El-Mallah, Maha Aboul-Ela, and Abdalla Ellakany. (2017). Evaluation of Analgesic Activity of Papaver libanoticum Extract in Mice: Involvement of Opioids Receptors.Evidence-Based Complementary and Alternative Medicine.2017(https://www.hindawi.com/journals/ecam/2017/8935085/#abstract)
20. Mohamed Eltaweel.Assessment of Antimicrobial Activity of Onion Extract (Allium cepa) on Staphylococcus aureus; in vitro study. International Conference on Chemical, Agricultural and Medical Sciences.Dec. 29-30, 2013,Kuala Lumpur, Malaysia, semanticscholar.org.
21. MI Ezeja, Ys Omeh, II Ezeigbo and A Ekechukwu. Evaluation of the Analgesic activity of the Methanolic Stem Bark Extract of Dialium guineense (Wild).Annals of Medical and Health Sciences Research.2011; 1(1): 55-62.
22. Ng, A., Parker, M. L., Parr, A. J., Saunders, P. K., Smith, A. C., & Waldron, K. W. Physicochemical characteristics of onion (Allium cepa L.) tissues. J Agric Food Chem,2000; 48(11): 5612-5617.
23. Onion;03/2003;www.selfsufficientish.com; Self Suffcientish; onlin; Onion; www.onions-usa.org; National Onion Association; onlinehttps://www.drweil.com/health-wellness/balanced-living/wellness-therapies/herbal-medicine/
24. Perianayagam JB, Sharma SK, Joseph A, Christina AJ. Evaluation of Anti-pyretic and Analgesic activity of Emblica officinalis Gaertn.J Ethnopharmacol.2004; Nov;95(1):83-5.
25. Punchard, N.A., Whelan, C.J. & Adcock, I. The Journal of Inflammation. J Inflamm 1, 1(2004). https://doi.org/10.1186/1476-9255-1-1
26. Rajesh Asija, Himanshu Baheti, Radhey Shyam Kumawat, Divya Sharma. Evaluation of Anti-inflammatory and Analgesic activity of Abrus precatorious leaves on Albino rats. IOSR journal of Pharmacy and Biological Sciences.2015; l 10, (5) Ver 1. www.iosrjournals.org.
27. Shinkafi,S.A and Dauda,H (2013). Antibacterial Activity of Allim cepa (onion) on some pathogenic Bacteria associated with Ocular infections. Scholar Journal of Applied Medical Sciences.Bol 2013;1(3):147-151.
28. Ajaykumar TV,Anandarajagopa K,Anbu Jeba Sunilson J, Adibah Arshad*, R.A.M.Jainaf, Venkateshanl N.Anti-inflammatory activity of Cosmos caudatus. international journal of universal pharmacy and bio sciences.2012;(2).
29. Vikas, V.P and Vijay, R.P. Evaluation of anti-inflammatory activity of Ficus carica Linn. Leaves. Indian Journal of Natural Products and Resources.2011;2(2): 151-155.
30. Zainul Amiruddin Zakaria,Loo Yi Wen, Nurul Izzah Abdul Rahman, Abdul Halim Abdul Ayub, Mohd. Roslan Sulaiman and Hanan Kumar Gopalan .Antinociceptive, Anti-Inflammatory and Antipyretic Properties of the Aqueous Extract of Bauhinia purpurea Leaves in Experimental Animals. Med Princ Pract 2007; 16:443–449.
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B, K., K. N. K, H. S. JC, A. K, J. Kaur, and S. Paramasivam. “EVALUATION OF ETHANOLIC ROOT EXTRACT OF ALLIUM CEPA. L FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES IN ANIMAL MODELS”. World Journal of Current Medical and Pharmaceutical Research, Vol. 2, no. 1, Feb. 2020, pp. 67-73, doi:10.37022/WJCMPR.2020.02017.
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Research Articles