SIMULTANEOUS ESTIMATION OF DUTASTERIDE AND SILODOSIN IN BULK FORM BY RP-HPLC METHOD

  • Kattempudi Paljashuva Chalapthi Institute of Pharmaceutical Sciences, Chalapthi Nagar, Lam, Guntur, AP India, 522.34.
  • a, Prof.N. Ramarao Chalapthi Institute of Pharmaceutical Sciences, Chalapthi Nagar, Lam, Guntur, AP India, 522.34.

Abstract

Dutasteride and Silodosin both are approved drugs by USFDA (Food & Drug Administration). On literature survey, it was found that few method have been reported for simultaneous estimation of Dutasteride and Silodosin. Therefore, it was thought of interest to develop a simple, accurate, precise, sensitive and economic analytical method and to validate as per ICH guidelines. So RP-HPLC method was developed and validated for simultaneous estimation of Dutasteride and Silodosin in multiunit system. Separation was achieved on Shimadzu HPLC; Agilent C18 Column (250×4.6mm,5µm ) by using a mobile phase containing Methanol : Water in 50:50 v/v ratio. Analysis was done at the filow rate of 1.0 ml/mint and PDA detection was carried out by wavelength at 280 nm. The retention time of Silodosin and Dutasteride was found to be 2.050 min & 2.623 min respectively. The method was validated in terms of linearity, precision, accuracy, specificity, robustness etc. The linearity was found to be in the range of 10-50 µg/ml for both Silodosin and Dutasteride with correlation coefficient of 0.999 for Silodosin and 0.999 for Dutasteride. %RSD of method precision was found to be less than 2%. This indicates that the method is precise.

Keywords: Method development, RP-HPLC, Validation

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How to Cite
Paljashuva, K., and a, P. Ramarao. “SIMULTANEOUS ESTIMATION OF DUTASTERIDE AND SILODOSIN IN BULK FORM BY RP-HPLC METHOD”. World Journal of Current Medical and Pharmaceutical Research, Vol. 1, no. 5, Jan. 2020, pp. 148-63, https://wjcmpr.com/index.php/journal/article/view/33.
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