Detection of Hbv Dna By Direct Pcr Protocol And Nested Pcr Protocol And Comparing Them. In Chronic Patients and Healthy Carriers of Hepatitis B Virus.

  • MushtaqTalib Al-Safi Ministry of Education, General Directorate of Education in Thi-Qar, Iraq

Abstract

Hepatitis B virus is a major public health problem, and the outcome of infection depends on the effectiveness of the virus's interaction with the host, and in particular on the strength of the natural and conditioned response of humoral and cellular immunity. This study was conducted to assess the clinical status, genetic immunity and viral (viral) traits of Iraqi patients with chronic hepatitis B virus and healthy carriers of hepatitis B surface antigen, as well as to show the relationship between the immune status and the chronic condition of the disease. The study included (60) patients with chronic hepatitis B virus type (B) and (60) healthy hepatitis B surface antigen carriers who attended the Teaching Hospital for Gastrointestinal and Liver Diseases in Nasiriya City Al-Hussein Teaching Hospital, Thalassemia Center and the Central Blood Bank in Nasiriya Citysouthern of Iraq. for the period from the first period. From February 2022 until the end of February 2022, the average age of patients infected with chronic hepatitis B virus was 64.5 years, and the average age of healthy carriers of hepatitis B surface antigen was 35. 1 year, and the ratio of males to females was (12.2:) for chronic patients and (13.6:) for carriers of the disease. As for the deoxyribonucleic acid, it was (100%) in chronic patients and healthy carriers using the Nested type polymerase chain reaction, and at a rate of (30.0%) and (46.7%) in chronic patients and healthy carriers, respectively using the polymerase chain reaction technique. direct with a significant difference between the two interactions. The current results confirm that a nested PCR protocol to detect HBV DNA is a more sensitive way of detecting HBV in Iraq CHB patients and carriers. Recent advanced techniques such as nucleic acid assays, quantitative assays for viral load and RT-PCR for Iraqi viral hepatitis laboratories are recommended.

Keywords: Hepatitis B virus, PCR protocol, HBV DNA, Nested PCR protocol

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Author Biography

MushtaqTalib Al-Safi , Ministry of Education, General Directorate of Education in Thi-Qar, Iraq

Ministry of Education, General Directorate of Education in Thi-Qar, Iraq

References

1. Aghdaie, S. F. A., Ansari, A., &Telgerdi, J. Analysing the impact of gamification dynamics and e-WOM on impulsive buying. International Journal of Procurement Management.2022, 15(1), 40-61.
2. Al-Fraiji, S. S., & Al-Shammary, D. Survey for Electroencephalography EEG Signal Classification Approaches. In Mobile Computing and Sustainable Informatics (pp. 199-214). Springer, Singapore, 2022.
3. Ali, H. M., Al-Shuwaikh, A. M., &Manuti, K. Molecular and phylogenetic detection of Torque Teno Virus (TTV) among hemodialysis patients: A single center study. Iraqi JMS.20(1): 11-25. doi: 10.22578. IJMS. 2022, 20(3).
4. Arankalle, V. A. 14 HEPATITISVIRUSES: VIROLOGY EPIDEMIOLOGY. Tropical Hepato-Gastroenterology-E-Book. 2008 , 261.
5. Arankalle, V. A. 14 HEPATITISVIRUSES: VIROLOGY AND. Tropical Hepato-Gastroenterology. 2010, 261.
6. Girish, C., & Pradhan, S. C. Drug development for liver diseases: focus on picroliv, ellagic acid and curcumin. Fundamental & clinical pharmacology. 2008,22(6), 623-632.
7. Groves, J. A., Y. Dodd, R., Zhu, X., Linnen, J. M., &Stramer, S. L. Background rate of low‐level HCV RNA in anti‐HCV confirmed‐positive and minipool nucleic acid test‐nonreactive blood donations. Transfusion. 2022, 62(1), 143-150.
8. Hong, J. M., Gibbons, M., Bashir, A., Wu, D., Shao, S., Cutts, Z., ...&Pawlosky, A.ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodes. Iscience. 2022, 25(1), 103586.
9. Horvat, R. T., & Taylor, R. Hepatitis B and D viruses. Manual of clinical microbiology. 2015,1841-1858.
10. Hu, F., Liu, Y., Zhao, S., Zhang, Z., Li, X., Peng, N., & Jiang, Z. A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics. Biosensors and Bioelectronics. 2022, 113994.
11. Pacin-Ruiz, B., Cortese, M. F., Tabernero, D., Sopena, S., Gregori, J., García-García, S., ...& Rodríguez-Frías, F. Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability. Viruses. 2022, 14(2), 215.
12. Pollicino, T., Amaddeo, G., Restuccia, A., Raffa, G., Alibrandi, A., Cutroneo, G., ... & Raimondo, G. Impact of hepatitis B virus (HBV) preS/S genomic variability on HBV surface antigen and HBV DNA serum levels. Hepatology. 2012, 56(2), 434-443.
13. Velikova, G., Booth, L., Smith, A. B., Brown, P. M., Lynch, P., Brown, J. M., & Selby, P. J. Measuring quality of life in routine oncology practice improves communication and patient well-being: a randomized controlled trial. Journal of Clinical Oncology. 2004, 22(4), 714-724.
14. Webb, G. W., Kelly, S., & Dalton, H. R. Hepatitis A and Hepatitis E: Clinical and epidemiological features, diagnosis, treatment, and prevention. Clinical Microbiology Newsletter. 2020, 42(21), 171-179.
15. Zahn, K. E., Jensen, R. B., Wood, R. D., &Doublié, S. Human DNA polymerase θ harbors DNA end-trimming activity critical for DNA repair. Molecular cell. 2021, 81(7), 1534-1547.
16. ZADEH, FiroozehAbolhasani, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. RendicontiLincei. ScienzeFisiche e Naturali, 2022, 1-7.‏
17. ROHMAH, Martina Kurnia, et al. Modulatory role of dietary curcumin and resveratrol on growth performance, serum immunity responses, mucus enzymes activity, antioxidant capacity and serum and mucus biochemicals in the common carp, Cyprinuscarpio exposed to abamectin. Fish & Shellfish Immunology, 2022, 129: 221-230.‏
18. ARIF, Anam, et al. The functions and molecular mechanisms of Tribbles homolog 3 (TRIB3) implicated in the pathophysiology of cancer. International Immunopharmacology, 2023, 114: 109581.‏
19. MARGIANA, Ria, et al. Functions and therapeutic interventions of non-coding RNAs associated with TLR signaling pathway in atherosclerosis. Cellular Signalling, 2022, 100: 110471.‏
20. H. A. Al-Hchaimi, M. F. Alhamaidah, H. Alkhfaji, M. T. Qasim, A. H. Al-Nussairi and H. S. Abd-Alzahra, "Intraoperative Fluid Management for Major Neurosurgery: Narrative study," 2022 International Symposium on Multidisciplinary Studies and Innovative Technologies (ISMSIT), 2022, pp. 311-314, doi: 10.1109/ISMSIT56059.2022.9932659.
21. MOHAMMED, Zainab; QASIM, Maytham T. The Relationship between Insulin Resistance and Hypertension in Patient with Hypertensive. HIV Nursing, 2022, 22.2: 1659–1663-1659–1663.‏
22. LEI, Zimeng, et al. Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor. Frontiers in Chemistry, 2022, 10.‏
23. BASHAR, Bashar S., et al. Application of novel Fe3O4/Zn-metal organic framework magnetic nanostructures as an antimicrobial agent and magnetic nanocatalyst in the synthesis of heterocyclic compounds. Frontiers in Chemistry, 2022, 10.‏
24. LAFTA, Holya A., et al. Tumor-Associated Macrophages (TAMs) in Cancer Resistance; Modula-tion by Natural Products. Current topics in medicinal chemistry, 2023.‏
25. AL-JASSANI, Mohammad J., et al. Isolation and Evaluation of Antibacterial Agents Produced by Soil Bacillus SP. and Study Some of their Immunological Parameters. Revista Electronica de Veterinaria, 2022, 23.4: 105-111.‏
26. SANE, Shahryar, et al. Investigating the effect of pregabalin on postoperative pain in non-emergency craniotomy. Clinical Neurology and Neurosurgery, 2023, 226: 107599.‏
27. Zhao, Z., Shang, P., Sage, F., &Geijsen, N. Ligation-assisted homologous recombination enables precise genome editing by deploying both MMEJ and HDR. Nucleic Acids Research, 2022.
Published
17-03-2024
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How to Cite
Al-Safi, M. “Detection of Hbv Dna By Direct Pcr Protocol And Nested Pcr Protocol And Comparing Them. In Chronic Patients and Healthy Carriers of Hepatitis B Virus.”. World Journal of Current Medical and Pharmaceutical Research, Vol. 6, no. 1, Mar. 2024, pp. 36-40, doi:10.37022/wjcmpr.v6i1.317.
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Research Articles