SYNTHESIS AND CHARACTERIZATION OF ZNO NANOPARTICLES USING LEAF EXTRACT OF RHIZOPHORA MUCRONATA AND EVALUATION OF THEIR ANTIMICROBIAL EFFICACY
Nanotechnology is a developing interdisciplinary field of research interspersing material science, bionanoscience, and technology. Nanoparticles are studied extensively for their specific catalytic, magnetic, electronic, optical, antimicrobial, wound healing and antiinflammatory properties. The main aim of the present study was to synthesize ZnO nanoparticles using the aqueous extract of Rhizophora mucronata leaves and to evaluate their antimicrobial efficacy against some selected microbes. The synthesis Zn nanoparticles were characterized by UV/VIS spectroscopy, particle size analyzer and Scanning Electron Microscopy. The synthesized Zn nanoparticles showed significant antimicrobial activity against Gram-positive and Gram-negative bacteria as well as against a fungal strain. .A clear zone inhibition was measured;40.05mm±0.137 for Staphylococcus aureus, and 36.15 mm ± 0.304 for Escherichia coli that comparably better result than a standard antibiotic. Thus from this study, it can be concluded that rhizophora mucronata extracts can be effectively used for synthesizing Zno nanoparticles. This study also suggests that green synthesized Zn nanoparticles can be used as an alternative to existing antimicrobial agents.
2. Awwd, A., Salem, N., Abdeen, AOMM. 2013. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Int. J. Indust. Chem., 4: 29 34.
3. Vennila, S., Jesurani, S. S., Priyadharshini, M., & Ranjani, M. (2016). Eco-friendly synthesis of metal oxide nanoparticles using Carissa carandas fruit extract. World Journal Pharmaceutical Research, 5, 806–812.
4. Ingle, A., Gade, A., Pierrat, S., et al. 2008. Psychosynthesis of silver nanoparticle using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria, Curr Nanosci.,4: 141 144
5. Rao, M., Savithramma, L.N. 2011. Biological synthesis of silver nanoparticles using Svensonia hydrabadensis leaf extract and evaluation of their antimicrobial efficacy. J. Pharm. Sci. Res., 3: 1117 1121.
6. Duran, N., Marcato, P.D., Alves, O.L., et al. 2005. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J. Nanotechnol., 3: 1 7.
7. Feris, K., Otto, C., Tinker, J., et al. 2010. Electrostatic interactions affect nanoparticle-mediated toxicity to the gram-negative bacterium Pseudomonas aeruginosa PAO. Langmuir, 26(6): 4429 36.
8. Guanabana, S., Sivaraja, R., Rajendran, V. 2012. Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. Prog. Natural Sci. Mater. Int., 22(6): 693 700.
9. Senthilkumar, S. R., & Sivakumar, T. (2014). Green tea (Camellia sinensis) mediated synthesis of Zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities. International Journal of Pharmacy and Pharmaceutical Sciences, 6, 461–465.
10. Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia, 1, 98 –106.
11. Vasudeo, Z., & Sonika, B. (2009). Antimicrobial activity of tea (Camellia sinensis). Biomedical & Pharmacology Journal, 2, 173–175.
12. Khandelwal, N., Singh, A., Jain, D., et al. 2010. Green synthesis of silver nanoparticles using Argemone mexicana leaf extract and evaluation of their antimicrobial activities. Dig. J. Nanomater. Bios., Pp. 483 489. Malabdi, R., Mulgund, G., Meti, N. et al. 2012.Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea. Res. Pharm., 2: 10 21. Rai, M., Yadav, A., Gade, A. 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv., 27: 76 83.
13. Russell, A.D., Hugo, W.B. 1994. Antimicrobial activity and action of silver. Prog. Med. Chem., 31: 351 370. Satyavani, K., Gurudevan, S., Ramanathan, T. et al. 2011. Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad. J. Nanobiotechnol., 9: 43.
14. Sosa, I.O., Noguez, C., Barrera, R.G. 2003. Optical properties of metal nanoparticles with arbitrary shapes. J. Phys. Chem. B., 107: 6269 6975. Sun, Y.G., Mayers, B., Herricks, T., et al. 2003. Polyol synthesis of uniform silver nanowires: a plausible growth mechanism and the supporting evidence. Nano Lett., 3: 955 960. Taylor, P.L. 2005. Ussher AL, Burrell RE. Impact of heat on nanocrystalline silver dressings. Part I: chemical and biological properties. Biomaterials, 26: 7221 7229. Vanaja, M., Gnanajobitha, G., Paul Kumar, K., et al. 2013. Phytosynthesis of silver nanoparticles by Cissus quadrangularis, influence of physicochemical factors. J. Nanostruct. Chem., 3: 17 24.
15. Zhang,L.,Ding,Y.,Povey,M.,&York,D.(2008).ZnO nanofluids – As Potential Antibacterial Agent. Progress in Natural Science,18,939–944.doi:10.1016/j.pnsc.2008.01.026