Isolation of Antibiotic Producing Microorganisms from Two Soil Samples of Phirangipuram,Guntur
Abstract
Antibiotics are chemicals, that kill or inhibit the growth of bacteria and are used to treat bacterial infections. They are produced in nature by soil bacteria and fungi. Soil is the outer region of earth crust consisting of loose material. In this, soil samples from two areas were collected (vegetable dump area(A), street soil(B)) and analysed, different bacterial colonies from soil samples was formed and tested for antimicrobial activity. The isolated and subcultured strains showed permanent zones of inhibition. The inhibitory activity of the organism was checked against important microbial flora. In the present study a trail was done to find out a new antimicrobial agent, producing bacteria from soil samples collected from two places.
Keywords:
Soil, Isolation, Antibiotics, Subculturing, Morphology, Staining
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References
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2. Jechalke, S., Heuer, H., Siemens, J.,Amelung, W.&Smalla, K.Fate and effects of veterinary antibiotics in soil. Trends Microbiol. 22, 536-545 (2014).
3. Gerits E, Blommaert E, Lippell A, ONeill A J, Weytjens B, Elucidation of the mode of action of a new antibacterial compound active against Staphylococcus aureus and Pseudomonas aeruginosa. PLoS One11(5) (2016).
4. Bizuye A, Moges F, Andualem B Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pac J Trop Dis 3(5): 375-381.
5. Kyselkova, M. Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure. Soil Biol. Biochem. 81, 259-265 (2015).
6. Shapiro E, Baneyx F Stress-based identification and classification of antibacterial agents: second generation Escherichia coli reporter strains and optimization of detection. Antimicrob Agents Chemother 46(8): 2490-2497 (2002).
7. Goh E, Yim G, Tsui W , McClure J, Surette MG Transcriptional modulation of bacterial gene expression by subinhibitory concentrations of antibiotics. Proc Natl Acad Sci USA 99(26): 17025-17030 (2002).
8. Fischer HP, Brunner NA, Wieland B, Paquette J, Macko L, Identification of antibiotic stress-inducible promoters: a systematic approach to novel pathway-specific reporter assays for antibacterial drug discovery. Genome Res 14(1): 90-98 (2004).
9. Michael J. Pelczar, JR, E.C.S. CHAN, Novel R.Krieg, MICROBIOLOGY, Fifth edition.
10. Dr. R. C. Dubey, Dr. D. K. Maheshwari S.CHAND, A Text book of Microbiology Revised edition.
11. Retinowati W Identification of Streptomyces sp-MWS1 producing antibacterial compounds. Indonesian J Trop Infect Dis 1(2): 82-85 (2010).
12. Kumar N, Singh RK and Mishra SK. Isolation and screening of soil actinomycetes as sources of antibiotics active against bacteria. International Journal of Microbiology Research. 2:12-16 (2010).
13. Agadagba SK. Isolation of Actinomycetes from soil. Journal of Microbiology Research. 4(3):136-140, (2014).
14. Baltz R.H.Microbe, 2, 125-131, (2007).
15. Newman D.J. and Cragg G.M. Nat Prod, 71, 461-477 (2007).
16. Haque SFK, Sen SK, and Pal SC. Screenining and identification of antibiotic produing strains of Streptomyces. Hindustan Antibiot Bull (3-4): 76-83.
17. Thmson JM, Bonomo RA. The threat of antibiotic resistence in Gram-negative bacteria: Beta-lactam in peril Curr Opin Microbial :8:518-24 (2006).
18. Goldstein DA, Tinland B, Gilbertson LA, Human safety and genetically modified plants: A review of antibiotic resistence markers and future transformation selection technologies. J Appl Microbiol 99:7-23 (2005).
19. Fierer, N. Embracing the unknown: disentangling the complexities of the soil microbiome. Nat. Rev. Microbiol. 15, 579-590 (2017).
20. Hakima, N, First case of neonatal bacteremia due to Dyella genus. Diagn. Microbiol, Infect Dis, 87, 199-201 (2017).
21. R Core Team. R: A Language and Environment for Statistical Computing. Available http://www.r-project.org/(2018).
22. Bertrand H, Poly F, Van VT. High molecular weight DNA recovery from soils prerequisite for biotechnological Meta genomic library construction. J Microbiol Methods;62:1-11 (2005).
23. Rigali, S.;Anderssen, S.;Naome, A.;van Wezel, G.P. Cracking the Regulatory Code of Biosynthetic Gene Clusters as a Strategy for Natural Product Discovery. Biochem. Pharmacol.(2018).
24. Stamatakis , A. RA×ML version 8: Atool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 1312-1313[PubMed] (2014).
25. Oliver, J.D. The viable but nonculturable state in bacteria. J. Microbiol. 43, 93-100 [PubMed], (2005).
26. Hibbing M, Fuqua C, Parsek M, Peterson S Bacterial competition: surviving and thriving in the microbial jungle. Nat Rev Microbial 8(1): 15-25 (2010).
Published
01-05-2020
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1.
C.H. NS, D RBR, K R, K LS, K VD, Bangaaru Lakshmi, Bangaaru Lakshmi. Isolation of Antibiotic Producing Microorganisms from Two Soil Samples of Phirangipuram,Guntur. World Journal of Current Med and Pharm Research [Internet]. 2020May1 [cited 2024Dec.6];2(2):211-3. Available from: https://wjcmpr.com/index.php/journal/article/view/128
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