PHYTOCHEMICAL SCREENING AND INVITRO ANTI-INFLAMATORY ACTIVITY OF ETHANOLIC EXTRACT OF Centella asiatica

  • Ms.Sini Baby Nirmala College of Pharmacy, Muvattupuzha, Ernakulam District, Kerala, India-686661.
  • Antony Varghese Antony Nirmala College of Pharmacy, Muvattupuzha, Ernakulam District, Kerala, India-686661.
    antonyantonyvarghese@gmail.com
  • Biniya John Nirmala College of Pharmacy, Muvattupuzha, Ernakulam District, Kerala, India-686661.
  • Dr.Deepa Jose Nirmala College of Pharmacy, Muvattupuzha, Ernakulam District, Kerala, India-686661.

Abstract

In ayurvedic medicine Centella asiatica is a valuable medicinal herbaceous creeper which has been valued for hundreds of years. Phytochemical analysis of Centella asiatica (Apiaceae) plant extracts revealed the presence of varied biochemical compounds like alkaloids, flavonoids, glycosides, phenolic compounds, triterpenoids and saponin etc. Since phenolic compounds, triterpenoids and flavonoids have remarkable anti-inflammatory, anti-arthritic and antioxidant activities, so our present work aims at evaluating the in vitro anti-inflammatory activity by Human Red Blood Cell (HRBC) membrane stabilization. To measure the anti-inflammatory activity, the inhibition of hypotonicity induced HRBC membrane lysis was used. The percentage Haemolysis was experimented from concentration of 50µg/ml to 2000µg/ml and the values reduced from 32.25% to 5.02%, on the other hand percentage Stabilisation in the same concentration range increased from 67.74% to 94.97%. Diclofenac sodium was used as the standard drug and the same experiment conducted in the same concentration range and the values of percentage haemolysis reduced from 47.18% to 1.24% and the percentage stabilisation increased from 52.81% to 98.76%. The results show that the extracts of Centella asiatica exhibited anti-inflammatory activities. Centella asiatica may be a profusely branched prostate herb consisting of active principles like Vallarine, Asiaticoside, Sitosterol, Tannins, Oxy asiaticoside. Asiaticoside is used in the treatment of leprosy. Sitosterol and tannin possess antiprotozoal and spasmolytic property. According to Siddha literature, the leaves of Centella asiatica are used for the treatment of syphilis, elephantiasis, all kinds of fever, abdominal disorder of children and hydrocele and these features are highlighted in this article.

Keywords: Centella asiatica, Anti-inflammatory, HRBC membrane stabilization

Downloads

Download data is not yet available.

References

1) Kumbhare MR, Sivakumar T, Lakhote T, Morankar PG. An evaluation of membrane stabilizing activity and antimicrobial activity of stem bark of Moringa oleifera (Moringaceae) against selected microbes. American Journal of Drug Discovery and Development. 2014;4:41-9.
2) Chippada SC, Volluri SS, Bammidi SR, Vangalapati M. In vitro anti-inflammatory activity of methanolic extract of Centella asiatica by HRBC membrane stabilisation. Rasayan J Chem. 2011;4(2):457-60.
3) Parameswari P, Devika R, Vijayaraghavan P. In vitro anti-inflammatory and antimicrobial potential of leaf extract from Artemisia nilagirica (Clarke) Pamp. Saudi journal of biological sciences. 2019 Mar 1;26(3):460-3.
4) Sivakrishnan S, Veeramani G. Phytochemical Activity Of Aerial Parts Of Cordia Obliqua Willd. International Research Journal of Pharmacy. 2019Feb;10(7):94–8.
5) Mamtha B, Kavitha K, Srinivasan KK, Shivananda PG. An in vitro study of the effect of Centella asiatica [Indian pennywort] on enteric pathogens. Indian Journal of Pharmacology. 2004 Jan 1;36(1):41.
6) Antony B, Santhakumari G, Merina B, Sheeba V, Mukkadan J. Hepatoprotective effect of Centella asiatica (L) in carbon tetrachloride-induced liver injury in rats. Indian Journal of Pharmaceutical Sciences. 2006;68(6):772.
7) Sarumathi A, Saravanan N. A study on the hematological parameters and brain acetylcholine esterase activity in immobilization induced stress and co-treatment with Centella asiatica leaves extract to wistar rats. International Journal of Nutrition, Pharmacology, Neurological Diseases. 2013 Apr 1;3(2):102.
8) Meena H, Pandey HK, Pandey P, Arya MC, Ahmed Z. Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica. Indian journal of pharmacology. 2012 Jan;44(1):114.
9) Raghavendra M, Maiti R, Kumar S, Trigunayat A, Mitra S, Acharya SB. Role of Centella asiatica on cerebral post-ischemic reperfusion and long-term hypoperfusion in rats. International Journal of Green Pharmacy (IJGP). 2009;3(2).
10) Dr Kokate C.K; Purohit A.P; Ghokhale S.B; Introduction in : Text book of Pharmacognosy, Nirali Prakashan, Vol.18,2002
11) Ayurvedic Encyclopedia- 3
12) Tripathi K..D Essentials of Medical pharmacology. 6th ed Jaypee Brothers Medical publishers (P)Ltd.: New Delhi; 2008
13) Ghani A. Medicinal plants of Bangladesh. The Asiatic Society of Bangladesh : Dhaka:2003
14) Natural Products, Raphel Ikan , 2nd Edition Comprehensive practical organic chemistry, V.k. Ahluwalia, Renu Aggarwal
15) V. Rajendran, K.S. Lakshmi., Bangladesh J Pharmacol., year 2008, Vol 3, Page No 121-124
16) M. N. A. Sreejayan Rao., J Pharm Pharmacol., Year 1997, Vol 49, Page No 105-107.
17) Kusanop .G. Orihara.S et al . , Chem.Pharm Bull,2002 Vol 50, 177-192
18) K. K. Kakkar., Indian Drugs., Year 1988, Vol 26, Page no 92-97.
19) Martindale , The extra Pharmacopeia
20) Indian Medicinal Plants - Author: Orient Longmann Page No.52
21) Handbook of Medicinal Plants - Author: Dr. P.N.V. Kurup Page No.61
22) Medicinal Plants Authors: Robert Bentley and Henry Trimen Page No:117
23) Medicinal Plants and Raw Drugs of India Authors: Purshotam Kaushik & Anil kumar dhiman
Statistics
52 Views | 38 Downloads
How to Cite
Ms.Sini Baby, M. B., A. V. Antony, B. John, and D. Jose. “PHYTOCHEMICAL SCREENING AND INVITRO ANTI-INFLAMATORY ACTIVITY OF ETHANOLIC EXTRACT OF Centella Asiatica”. World Journal of Current Medical and Pharmaceutical Research, Vol. 2, no. 2, May 2020, pp. 181-3, doi:10.37022/WJCMPR.2020.2218.
Section
Research Articles